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electron microscope zeiss 10 9  (Carl Zeiss)


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    Carl Zeiss electron microscope zeiss 10 9
    Effect of silencing of gC1qR gene on TcdA-induced cell apoptosis in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Cell viability was determined by WST-1 assay as described previously; b Apoptotic death of NCM 460 cells was examined by flow cytometric analysis. The data are means ± S.D. of three separate experiments performed in triplicate. c The expression of caspase-3 protein in lysates of NCM460 cells was measured by western blot assay. *** p < 0.001, ** p < 0.01, * p < 0.05, # p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group (Mock group); △△ p < 0.01 versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲ p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group. d The change of morphology of the NCM 460 cells was observed with an electron <t>microscope.</t> Red arrows point to the nuclei condensed and fractured, and chromatin increased and marginalized in the nucleus (3700 X)
    Electron Microscope Zeiss 10 9, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electron microscope zeiss 10 9/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    electron microscope zeiss 10 9 - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "The role of the globular heads of the C1q receptor in TcdA-induced human colonic epithelial cell apoptosis via a mitochondria-dependent pathway"

    Article Title: The role of the globular heads of the C1q receptor in TcdA-induced human colonic epithelial cell apoptosis via a mitochondria-dependent pathway

    Journal: BMC Microbiology

    doi: 10.1186/s12866-020-01958-6

    Effect of silencing of gC1qR gene on TcdA-induced cell apoptosis in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Cell viability was determined by WST-1 assay as described previously; b Apoptotic death of NCM 460 cells was examined by flow cytometric analysis. The data are means ± S.D. of three separate experiments performed in triplicate. c The expression of caspase-3 protein in lysates of NCM460 cells was measured by western blot assay. *** p < 0.001, ** p < 0.01, * p < 0.05, # p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group (Mock group); △△ p < 0.01 versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲ p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group. d The change of morphology of the NCM 460 cells was observed with an electron microscope. Red arrows point to the nuclei condensed and fractured, and chromatin increased and marginalized in the nucleus (3700 X)
    Figure Legend Snippet: Effect of silencing of gC1qR gene on TcdA-induced cell apoptosis in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Cell viability was determined by WST-1 assay as described previously; b Apoptotic death of NCM 460 cells was examined by flow cytometric analysis. The data are means ± S.D. of three separate experiments performed in triplicate. c The expression of caspase-3 protein in lysates of NCM460 cells was measured by western blot assay. *** p < 0.001, ** p < 0.01, * p < 0.05, # p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group (Mock group); △△ p < 0.01 versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲ p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group. d The change of morphology of the NCM 460 cells was observed with an electron microscope. Red arrows point to the nuclei condensed and fractured, and chromatin increased and marginalized in the nucleus (3700 X)

    Techniques Used: Transfection, WST-1 Assay, Expressing, Western Blot, Microscopy



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    Effect of silencing of gC1qR gene on TcdA-induced cell apoptosis in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Cell viability was determined by WST-1 assay as described previously; b Apoptotic death of NCM 460 cells was examined by flow cytometric analysis. The data are means ± S.D. of three separate experiments performed in triplicate. c The expression of caspase-3 protein in lysates of NCM460 cells was measured by western blot assay. *** p < 0.001, ** p < 0.01, * p < 0.05, # p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group (Mock group); △△ p < 0.01 versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲ p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group. d The change of morphology of the NCM 460 cells was observed with an electron <t>microscope.</t> Red arrows point to the nuclei condensed and fractured, and chromatin increased and marginalized in the nucleus (3700 X)
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    Effect of silencing of gC1qR gene on TcdA-induced cell apoptosis in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Cell viability was determined by WST-1 assay as described previously; b Apoptotic death of NCM 460 cells was examined by flow cytometric analysis. The data are means ± S.D. of three separate experiments performed in triplicate. c The expression of caspase-3 protein in lysates of NCM460 cells was measured by western blot assay. *** p < 0.001, ** p < 0.01, * p < 0.05, # p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group (Mock group); △△ p < 0.01 versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲ p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group. d The change of morphology of the NCM 460 cells was observed with an electron microscope. Red arrows point to the nuclei condensed and fractured, and chromatin increased and marginalized in the nucleus (3700 X)

    Journal: BMC Microbiology

    Article Title: The role of the globular heads of the C1q receptor in TcdA-induced human colonic epithelial cell apoptosis via a mitochondria-dependent pathway

    doi: 10.1186/s12866-020-01958-6

    Figure Lengend Snippet: Effect of silencing of gC1qR gene on TcdA-induced cell apoptosis in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Cell viability was determined by WST-1 assay as described previously; b Apoptotic death of NCM 460 cells was examined by flow cytometric analysis. The data are means ± S.D. of three separate experiments performed in triplicate. c The expression of caspase-3 protein in lysates of NCM460 cells was measured by western blot assay. *** p < 0.001, ** p < 0.01, * p < 0.05, # p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group (Mock group); △△ p < 0.01 versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲ p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group. d The change of morphology of the NCM 460 cells was observed with an electron microscope. Red arrows point to the nuclei condensed and fractured, and chromatin increased and marginalized in the nucleus (3700 X)

    Article Snippet: The ultrastructure of NCM 460 cells was examined at 3700 X magnification, and photographs were observed under a Zeiss 10 9 electron microscope (Carl Zeiss, Oberkochen, Germany).

    Techniques: Transfection, WST-1 Assay, Expressing, Western Blot, Microscopy